“....this technique enables one to monitor online cell concentrations immobilised cultures and offers the potential to form the basis of control systems for cell growth and productivity optimisation”

Serono Biotech Center & Swiss Federal Institute of Technology
Request a full copy of paper 100
Ducommun P., Kadouri A., Von Stockar U, Marison I.W., On-line Determinations of Animal Cell Concentration in Two Industrial Processes by Dielectric Spectroscopy. Biotechnology and Bioengineering, Vol 77, No 3, February 5th, 2002

“The use of the capacitance probe can successfully replace the former measurements (cell dry weight, packed mycelial volume, viscosity, DNA concentration and total CO2 evolution) giving an accurate and online description of the biomass profile during the entire process time. Moreover it enables the online computation of the specific growth rate and thus the development of more accurate and reproducible process operational control strategies”

CIPAN & Instituto Superior Technico, Portugal
Request a full copy of paper 85
Neves A., Pereira D., Vieira L., Menezes J., Real time monitoring biomass concentration in Steptomyces clavuligerus cultivation with industrial media using a capacitance probe

“Capacitance measurement is a very good tool to observe the attachment of the cells on the carriers in real time. This offers the possibility to optimise the selection of the carriers (diameter, size of the pores, kind of modification) to obtain the best conditions for the cultivated cells”

Institute of Biotechnologie, Forschungszentrum Julich GmbH
Request a full copy of paper 76
Knoll, T., & Biselli, M. Dielectric Spectroscopy in the Cultivation of Suspended and Immobilised Hybridoma Cells. Journal of Biotechnology (1998) Vol 63 pp. 187-198

“The Biomass Monitor is a tool for analysing the evolution of the amount of living organisms during the process and to detect any change of its physiological state. It will be used as a new source of information for Virginiamycin process improvement”

Innovation Cell, Pfizer Antibiotics Plant Rixenstart
Request a full copy of paper 69
Innovation Cell, Pfizer Antibiotics Plant Rixenstart J-M Coremans, V.Joly, Ph. Dehottay and F.Gossele, Innovation Cell, Pfizer Antibiotics Plant Rixenstart, rue de l’ institute 87A, 1330 Rixensart, Belgium

“The Biomass Monitor allowed us to detect a phenomenon previously unreported in insect cell cultures infected with baculovirus ”

Animal Cell Technology and Downstream Processing Group, Biotechnologie Research Institute, NRC Canada
Request a full copy of paper 78
Zeiser, A., Bédard, C., Voyer, R., Jardin, B., Tom, R., & Kamen, R. R., On-line Monitoring of the Progress of Infection in Sf-9 Insect Cell Cultures Using Relative Permittivity Measurements. Biotechnology and Bioengineering, Vol. 63, No 1, pp.122-126 April 5th 1999

“the capacitance signal was stable and strongly related to changes in biomass concentration of both bacteria and yeasts. This instrument could therefore be used to monitor and control the growth of micro-organisms. Typical examples of use are: monitoring growth profiles, calculating online specific growth rates, adding pre-cursors within timed events, determining the onset of stationary phase / cell lysis etc. The instrument was particularly useful in following mycelial growth under industrial conditions for which precise offline measurements did not exist (PMV, X, OD). Under these circumstances, the instrument gave data which were closer to physiological reality and could be interpreted more easily than the traditional off-line measurements ”

Smithkline Beecham Biologicals, Rixensart Belghium & Ecole Superiuere de Biotechnologie de Strasbourg
Request a full copy of paper 25
Fehrenbach, R. – Ecole Superiuere de Biotechnologie de Strasbourg , Comberbach, M. & Pêtre, J.O. – Smithkline Beecham Biologicals, Rixensart Belgium